Cross-reactions of anti-IspC MAbs with other <i>L. monocytogenes</i> serotypes.

aIsolate details can be found in Table S1. bThe interaction between each antibody-isolate pair was examined using ELISA in three independent experiments. Positive reactions were recorded if the average of the three OD414 measurements was >25% of the OD414 recorded for the same antibody when reacting with L. monocytogenes serotype 4b strain LI0521. cNegative reactions are reported if the average OD414 of the three independent experiments was <25% of the OD414 recorded for the same antibody when reacting with L. monocytogenes serotype 4b strain LI0521. In most cases a negative reaction could also be defined by OD414 of all three experiments being below the 25% threshold. However, M2777, M2781, M2792, M2795, M2797 and M2799 each frequently had one or two of three the measurements above the 25% threshold, even though the average remained below 25%. The variability of each of these MAbs makes them poor candidates for diagnostics.