Sigrun M. Gustafsdottir, Vebjorn Ljosa, Katherine L. Sokolnicki, Joshua A. Bittker, Nicole E. Bodycombe, Mark Anthony Bray, Vlado Dancik, Tom Hasaka, C. Suk Yee Hon, Melissa M. Kemp, Kejie Li, Deepika Walpita, Mathias J. Wawer, Todd R. Golub, Stuart L. Schreiber, Paul A. Clemons, Anne E. Carpenter, Alykhan F. Shamji (2016) CIL:49189, Homo sapiens. CIL. Dataset

Plate 24310 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the cell-painting assay). This experiment consists of 375 plates in 384-well format, for a data set comprised of 988,994 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are approximately 5 million image files in 16-bit TIFF format.

本数据集编号为24310，旨在对每个样本的细胞表型进行多参数、基于特征的剖析，以使不同样本的表型之间的一致性反映样本本身的一致性。该图像集为测试基于图像的剖析方法提供了基础，以检验其区分小分子效应的能力。图像展示了经过1600种已知生物活性化合物处理的U2OS细胞，并使用六种标签对七个细胞器（细胞绘画实验）进行标记。该实验包括375个384孔板，数据集包含988,994个视野。每个视野在五个通道（检测波长）中进行成像，每个通道存储为单独的灰度图像文件，因此大约有500万个图像文件，格式为16位TIFF。