Effect of Abscissic acid on Brassica napus guard cells

Plants acclimate to drought and water stress through diverse physiological responses, primarily mediated by the hormone abscisic acid (ABA). The closure of stomatal pores on aerial surfaces of plants is one of the rapid responses mediated by ABA to reduce transpirational water loss because plants lose the majority of their water through stomatal pores. Stomatal guard cells have been an important cellular system for studying ABA signaling. The dynamic changes in the transcriptome of stomatal guard cells in response to ABA have been investigated in the model plant Arabidopsis thaliana. In this study, we analyzed the dynamics of ABA-regulated transcriptomes in stomatal guard cells of Brassica napus. Overall design: Guard cells were exposed to abscisic acid for 0 (control), 15, and 60 minutes for 3 replicates each. Grant Title: Cell-type Specific Networks in Brassica Guard Cell Responses to Drought Grant Award number: 1025837 Grantee: June Kwak Funding Agency: NSF