Detection of hiPSCs spiked into human neurons on the culture system using laminin-521 and Essential 8 medium.

Morphologies of forming colonies derived from 253G1 cells spiked into human neurons are shown (images in the left). 253G1 cells (1%, 1000 cells; 0.1%, 100 cells; 0.01%, 10 cells; 0%, 0 cells) were spiked into human neurons (100,000 cells) and co-cultured on laminin-521-coated wells in 12-well plates in Essential 8 medium for 6 days. Forming colonies were stained with anti TRA-1-60 antibody (images in the right). HNs, human neurons. Scale bars, 500 µm.