Gene Expression Signature in Rat Pituitary MMQ Cells Treated With DRD2 agonist UNC9994

DRD2 agonists are effective in treating pituitary tumors. To fathom the ß-arresin-dependent mechanisms underlying DRD2-mediated pituitary tumor growth suppression,we applied RNA-seq analysis to rat pituitary MMQ cells treated with a DRD2 ß-arresin-biased agoinst, UNC9994.MMQ cells were treated with UNC9994(15µM) or vehecle control for 12 h or 24 h and then subject to RNA-seq analysis.We found oxidative-stress-related genes such as Nqo1 and Hmox1 were upregulated by UNC9994, revealing oxidative stress may be the basis of UNC9994-induced tumor growth suppression. Our study represents the first detailed analysis of transcriptomes in rat pituitary MMQ cells treated with DRD2 ß-arresin-biased agonist.The significance of altered expression of specific transcripts will enhance our understanding of DRD2 signaling in pituitary tumor cells. Overall design: MMQ cells were treated with UNC9994 (15µM) or vehicle control (DMSO) for 12h or 24h