小鼠DNA 6mA识别蛋白MPND及其结合DNA复合物的X-射线成像数据集

"DNA 6mA生成和去除分别是由Mettl4甲基转移酶和ALKBH4双加氧酶催化。基因中6mA的积累，会造成相关传感器蛋白--组蛋白H2A去泛素化酶ASXL1和MPND的水解破坏，减少转录沉默。小鼠Mettl4的失活降低6mA含量并且在后代中引起亚致死性和颅面畸形，鉴定了能够从抑制性标记--组蛋白H2A（H2A-K119Ub）中去除单泛素化的MPND去泛素化酶和Polycomb抑制性去泛素化酶（PR-DUB）复合物的ASXL1组分的6mA传感器结构域。
本数据集描写的是小鼠DNA 6mA识别蛋白MPND及其结合DNA复合物的X-射线衍射数据，分辨率分别在2.06Å和2.47 Å。发现MPND直接与组蛋白结合，DNA进一步促进了它们的结合，这为研究基因激活和沉默途径提供了新的策略。因此本课题首次发现了MPND直接结合组蛋白以及酸性区域和DNA具有协同作用。MPND 的动态组装和独特特征为研究表观遗传学、基因控制和转录调控开辟了新途径。"

The generation and removal of DNA N6-methyladenosine (6mA) are catalyzed by the methyltransferase Mettl4 and dioxygenase ALKBH4, respectively. Accumulation of 6mA in genes triggers hydrolytic degradation of the relevant sensor proteins—specifically the histone H2A deubiquitinases ASXL1 and MPND—thereby attenuating transcriptional silencing. Inactivation of murine Mettl4 reduces genomic 6mA levels and induces sub-lethality and craniofacial malformations in offspring; this study further identified the 6mA sensor domain of ASXL1, a component of the Polycomb repressive deubiquitinase (PR-DUB) complex, and MPND deubiquitinase, which can remove monoubiquitination from the repressive histone mark H2A (H2A-K119Ub).

This dataset presents X-ray diffraction data of the murine DNA 6mA recognition protein MPND and its DNA-bound complexes, with resolutions of 2.06 Å and 2.47 Å, respectively. This work found that MPND directly binds to histones, and DNA further enhances this interaction, providing a novel approach for investigating gene activation and silencing pathways. Therefore, this study is the first to report that MPND directly binds histones, and that its acidic region and DNA exert synergistic effects. The dynamic assembly and unique properties of MPND open new avenues for research in epigenetics, gene regulation, and transcriptional control.

搜集汇总

背景与挑战

背景概述

该数据集提供了小鼠DNA 6mA识别蛋白MPND及其与DNA复合物的X-射线成像数据，分辨率分别为2.06Å和2.47Å，包含7个文件，总数据量4.09MB。数据集揭示了MPND作为6mA传感器直接结合组蛋白，且DNA促进这种结合，为研究表观遗传学和基因调控提供了新策略，首次发现MPND的动态组装和协同作用特征。