Early-Life 2'-Fucosyllactose and Its Combination with Bifidobacterium bifidum TMC3115: Sex-specific Effects in Learning, Spatial Memory and Hyperactivity in Juvenile SHR as an ADHD model

The supplementary files include:

Method: 5-choices serial reaction time task (5-CSRTT)
Method: Morris water maze (MWM)
Method: Real-time quantitative PCR (qPCR)
Method: Flow cytometry for Treg cell detection

Table S 1 Main composition of the rat feed (%)
Table S 2 Parameters of 5-CSRTT test
Table S 3 The locations the rats put into the water in MWM
Table S 4 The reverse-transcription PCR protocol
Table S 5 The qPCR protocol
Table S 6 The primer sequences
Table S 7 Relative abundance of gut microbiota at genus level of male rats (%)
Table S 8 Relative abundance of gut microbiota at genus level of female rats (%)
Table S 9 Function predictions of gut microbiota of male rats at 3w and 7w
Table S 10 Function predictions of gut microbiota of female rats at 3w and 7w

Fig. S 1 5-CSRTT of male rats and female rats
Fig. S 2 α diversities and PCoA maps of β diversity of male and female rats
Fig. S 3 Effects of Early life 2’-FL with or without B. bifidum TMC3115 intervention on the number of activated microglia in the prefrontal cortex, the number of Treg cells in MLN, spleen and blood, and the mRNA expression of tight junction proteins in the striatum and colon of male rats
Fig. S 4 Effects of Early life 2’-FL with or without B. bifidum TMC3115 intervention on the number of activated microglia in the prefrontal cortex, the number of Treg cells in MLN, spleen and blood, and the mRNA expression of tight junction proteins in the striatum and colon of female rats
Fig. S 5 Effects of early life 2’-FL and synbiotic intervention on the relative abundance of Bifidobacterium and Bacteroides between male and female SHR