Small RNA sequencing of virus-infected barley plants

To investigate the function of HvSDN1 in regulating the BYDV derivative vsiRNA accumulation, the barley plants with silencing HvSDN1 using BSMV-VIGS (BSMV-SDN1gs) and its control plants (BSMV-EV) were further employed to inoculate the BYDV-GAV, thus producing four groups of plants, i.e., BSMV-EV/BYDV- (without HvSDN1 silencing and free of BYDV), BSMV-SDN1gs/BYDV- (with HvSDN1 silenced and free of BYDV), BSMV-EV/BYDV+ (without HvSDN1 silencing and infected by BYDV), BSMV-SDN1gs/BYDV+ (with HvSDN1 silenced and infected by BYDV). The small RNAs were extracted from the four types of plants at 21 dpi of BYDV and were used to construct the small RNA-Seq library.