RNA-Sequencing to identify the transcriptional targets of Rai1

The goals of this study are to harvest adult-onset Rai1-deficient mouse brains from hypothalamus and compare the transcriptome with their wild-type littermates. Methods: mRNA profiles from Rai1 conditional knockouts and wild-type mice were generated by deep sequencing, in duplicate using Illumina system. Conclusions: Our study suggests that Rai1 preferentially regulate genes involved in cell communication Overall design: Hypothalamic mRNA profiles of wild-type and Rai1 conditional knockout mice were generated by deep sequencing, in duplicates.