ChIP-seq with DNA methylation inhibitor in HCT116. ChIP-seq with DNA methylation inhibitor in HCT116

Heterochromatin conformation is essential for endogenous retroviruses (ERVs) silencing. However, extensive maps of heterochromatin modifications H3K9me2/3 at ERV regions have not yet been provided in human cancer cells. Our ChIP-seq results showed that ERVs tend to be concentrated in regions of heterocromatin, which are marked by the presence of H3K9me3. We also observed dramatic increases H3K9me2/3 in the ERVs after transient treatment with DNA methylation inhibitor. Overall design: ChIP-seq using H3K9me2 and H3K9me2 antibodies were performed in the human colon cancer cell line HCT116, following DNA methylation inhibitor treatment.