Single-cell transcriptome of myeloid progenitor populations from the mouse bone marrow

Various types of myeloid cells play a universal and vital role in maintaining homeostasis and immune responses in vertebrates. However, the development of myeloid lineage diversity remain elusive. Here, we constructed a single-cell transcriptional map of myeloid progenitors from the mouse bone marrow, in order to dicepher the ontogeny of myeloid lineage diversity. The profiled myeloid progenitor samples include LMPP, CMP and three biological replicates of GMP. LMPP (Lin-c-Kit+Sca-1+CD34+Flt3+), CMP (Lin-c-Kit+Sca1-CD34+CD16/32low), and three biological replicates of GMP (Lin-c-Kit+Sca-1-CD34+CD16/32high) samples from the bone marrow of Balb/C mice were isolated and transcriptionally profiled by scRNA-seq (10x Genomics).