RNA-seq analysis on the liver of darkness rats treated with Limosilactobacillus reuteri

We have previously demonstrated that the arrhythmic expressions of circadian clock genes due to constant darkness induce glycometabolic and reproductive hallmarks of polycystic ovary syndrome (PCOS) in rats. Limosilactobacillus reuteri (L.reuteri) is a promising dietary intervention for host dysmetabolism, while its potential effect on circadian dysrhythmia-induced PCOS remains elusive. Here, we evaluated the amelioration of L.reuteri regimen on constant darkness-induced PCOS-like rats through detecting hepatic gene expression profiles by RNA-seq.我们此前已证实，持续黑暗可导致大鼠生物钟基因的节律异常表达，进而诱导其出现多囊卵巢综合征（polycystic ovary syndrome, PCOS）的糖代谢与生殖标志性特征。罗伊氏乳杆菌（Limosilactobacillus reuteri, L.reuteri）是一种颇具应用前景的宿主代谢紊乱膳食干预手段，但其对昼夜节律紊乱诱导的多囊卵巢综合征的潜在作用仍尚不明确。本研究通过RNA-seq检测肝脏基因表达谱，评估了罗伊氏乳杆菌干预方案对持续黑暗诱导的多囊卵巢综合征样大鼠的改善作用。