Figure S1 - Binding Properties and Stability of the Ras-Association Domain of Rap1-GTP Interacting Adapter Molecule (RIAM)

Analysis of the tryptic peptides and correlation with the protein sequence. The results of mass spectrometric analysis of tryptic peptides from the fragments generated by limited proteolysis (arrows in Figure 3B and C) are shown for the RIAM1-261, spanning from the N-terminus to the end of the RA domain, and RIAM150-502, including the RA-PH domain pair. In each panel, the upper box shows the identified peptides under the input sequence. The lower print lists the observed and calculated peptide masses and sequences. Note that the sequence numbering in the upper box starts from the beginning of the construct. Both constructs have a vector-derived sequence GAMG at the N-terminus. (PDF)