Acute pain after total knee arthroplasty: 2-arachidonoylglycerol tone and endocannabinoid/eicosanoid crosstalk

Background: Total knee arthroplasty (TKA) is a surgical procedure that induces intense acute postoperative pain, but the mechanisms that amplify post-TKA pain remain incompletely understood. Endocannabinoids, such as 2-arachidonoylglycerol (2-AG), are endogenous lipids that can produce antinociceptive effects. However, hydrolysis of 2-AG by monoacylglycerol lipase (MAGL) generates arachidonic acid, the precursor to a host of eicosanoids that enhance pain. The presence of this metabolic pathway suggests that individuals with elevated 2-AG levels may be primed to develop greater postoperative pain.
Methods: The primary goal of this prospective study was to determine if intraoperative 2-AG levels in the synovial fluid (SF) and cerebrospinal fluid (CSF) of TKA patients are associated with the magnitude of acute postoperative pain at rest and with ambulation. The secondary goal was to determine whether 2-AG metabolism contributes to prostaglandin E2 (PGE2) biosynthesis in synovial tissue ex ..., , , # Acute pain after total knee arthroplasty: 2-arachidonoylglycerol tone and endocannabinoid/eicosanoid crosstalk

Dataset DOI: [10.5061/dryad.tmpg4f584](10.5061/dryad.tmpg4f584)

## Description of the data and file structure

Intraoperative 2-AG levels in synovial fluid and cerebrospinal fluid of patients undergoing total knee arthroplasty.

Preoperative and postoperative patient-reported pain.

Prostaglandin E2 (PGE2) levels in the synovial fluid of patients undergoing total knee arthroplasty.

2-arachidonoylglycerol (2-AG), arachidonic acid (AA), and PGE2 levels in synovial explants treated with vehicle or 10 uM MJN110.

### Files and variables

#### File: Endocannabinoids_data_repository.xlsx

**Tab:** Data

**Description:** Preoperative pain, postoperative pain, and intraoperative 2-AG levels in synovial fluid and cerebrospinal fluid of patients undergoing total knee arthroplasty (TKA).

##### Variables

* Sex
* Age
* Body mass index (BMI)
* Koos Jr interval pain scores
* Numeric ra..., Explicit consent was provided from participants to publish the de-identified data in the public domain. Subjects were de-identified and were assigned a study number representing their data.