Images from Chandler, Chari et al. 2017 PLoS Genetics "How well do you know your mutation? Complex effects of genetic background on expressivity, complementation, and ordering of allelic effects"
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These are the images associated with the data collected for http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007075For the zipped folder GS_data:Data Provenance (how data was collected):via dissections, area imagingAuthor: Performed - Ian Dworkin, Gayatri Sivaratnam, Samiksha Kaul Dissections - Gayatri Sivaratnam, Samiksha KaulImaging - I. Dworkin, G. Sivaratnam, Samiksha Kaul Dates samples Collected: July 21, 2015Dissection: August 17 - September25, 2015Imging: September 30, 2015 (ID, GS, SK)(Date samples were collected, dates for imaging)A bit about the experiment:16 DGRP lines were crossed to 3 mutations (sd 1, sd E3, sd 58d) and a wildtype (SAM)The effects of intermediate mutation on different lines, whether the severity of this effect varied across the different lines is being investigated.Microscope, camera used. Leica m125 with a Leica DFC400 camera, using Leica Application Software 3.4.0. Greyscale images. Each image is 1392 x 1040 px. Real size 2.59 x 1.93 mmMagnification : 50X (0.5X?)aperture about halfway across (59%)Brightness: 68%Gamma: 0.80Notes:naming convention for images: SAM/sd1/sde3/sd58d_line#_M_1/2/3/4/5/6.TIFSAM/sd1/sde3/sd58d - either the wild type (SAM) allele for scalloped, or allele 1, E3 or 58d
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2018-03-02



