Transgenerational exposure to deoxygenation and warming disrupts mate detection in Gammarus locusta

Ocean deoxygenation and warming have been shown to pose a growing threat to the health of marine organisms and ecosystems. Yet, the potential for acclimation and adaptation remains poorly understood. The aim of this study was to evaluate the effects of transgenerational exposure to reduced oxygen availability and elevated seawater temperature on the chemosensory-dependent mating mechanisms of male amphipods Gammarus locusta. Three subsequent generations were exposed to four experimental treatments for 30 days: i) present-day scenario, ii) warming; iii) deoxygenation; and iv) warming+deoxygenation. After exposure, the number of individuals that reached adulthood was gauged, and adult males from F0 and F1 were subjected to behavioral trials to assess their capacity of long-distance female cue detection through quantification of response time, first direction of movement, activity rate, and proportion of time spent in female scent cues. Ocean-warming-induced mortality and reduced oxygen av..., Our study aimed to investigate the transgenerational effects of increased temperature and lower oxygen levels on male gammarids' response to female scent signals. For this purpose, we conducted a binary-choice experiment using a total of 30 males from the F0 generation and 26 males from the F1 generation. These trials involved direct observations, during which we recorded four key traits of male individuals: response time (time to initiate movement towards an association zone), proportion of first choice (initial movement towards the female-scented association zone), cumulative time spent in each association zone, and activity rate (proportion of time spent actively moving). 
Data analysis of behavioral outputs was performed through generalized linear mixed models (GLMM) using the negative binomial (number of individuals, response time), binomial (first choice), and Gaussian (cumulative time & activity rate) residual distributions. All models used temperature, oxygen, and generation..., , # Transgenerational exposure to deoxygenation and warming disrupts mate detection in Gammarus locusta

This dataset comprises a code script (script.rmd) with the associated raw data (data*.csv), used in the data analysis; and the water parameters registered daily in each replicate tank (parameters.xlsx).

## Description of the data and file structure

Data_beh.csv and Data_population.csv: to be imported into the R software, using the R script and changing the directory (setwd()) to where the data file is located.

Data_beh.csv: Data from behavioral trials (i.e., long-distance female cue detection) with male gammarids from two successive generations (F0 & F1) and four treatment groups varying in temperature (18 °C & 21 °C) and oxygen levels (90 % & 100 % air saturation).

Variables (Data_beh.csv):

* Temperature; temperature treatment in degrees celcius (18ºC - Control, 21ºC - Warming)
* Oxygen; air saturation (100% - Control, 90% - Deoxygenation)
* ID; replicate tank...