S6B_qPCR_let-7_lin-46_timecourse

Raw qPCR data supporting the Fig. S6B. Expression levels of mature let-7 miRNA (left) and lin-46 mRNA (right) measured by RT-qPCR in animals grown at 25°C and collected hourly from 12 to 17 hours of development. Expression levels at each time point were first normalized to the levels of a reference gene (small nucleolar RNA sn2841 for let-7, actin mRNA for lin-46) and then to wild type at 12 h. Condition Strain code Genotype wild type N2 wild type wild type* HW1792 lin-28(xe59[lin-28::flag::ha]) I lin-28(0) HW2705 lin-28(xe192) I lin-28(∆b) HW3381 lin-28(xe344xe59[lin-28::flag::ha with exon1B(Δ)]) I lin-28::large_tag HW2805 lin-28(xe197[lin-28::linker::aid::3xflag::tev::gfp::linker::hibit]) I