Genome-wide analysis reveals porcine LIFR regulated by DNA methylation promotes the implantation process via the STAT3 signaling

Here, we investigated the genome-wide DNA methylation landscape in the Yorkshire and Meishan pig endometrium. Endometrial DNA was isolated from Meishan pigs and Yorkshire pigs ondays 18 and 32 of pregnancy in equal amounts to generate eight DNA libraries (YK1801, YK1802, YK3201, YK3202, MS1801, MS1802, MS3201, and MS3202). To decipher the pig DNA methylome, we studied whole-genome DNA methylation based on immunoprecipitation using an antibody specific to 5 methylcytosine, followed by Illumina HiSeq 2000 sequencing as per the manufacturer's protocol (BGI, Shenzhen, China).