Gene expression signatures of cancer-associated fibroblasts in non-small cell lung cancer

To gain insight into the differential gene expression to aberrant fibroblast activation of the TME, we isolated primary CAFs and NFs from human NSCLC tissues and paired normal tissues,and performed microarray screening of CAFs and NFs CAFs and NFs were isolated from human NSCLC tissues and paired normal tissues (more than 5 cm away from the tumor margin). Freshly isolated surgical specimens were acquired from three patients. Fibroblast populations were identified by morphology, western blotting and immunofluorescence staining.