CGH array used to identify de novo copy number variations in offspring of BaP exposed parents.

CGH arrays were used to detect de novo CNVs in the litters produced from mating MutaMouse sires with C57Bl/6J dams. Specifically, high molecular weight DNA was extracted from the liver of each animal. One µg of offspring DNA was labelled with Cy5 dye. Five hundred ng of DNA from each respective parent were mixed together and labelled with Cy3 dye. As positive controls, and to identify existing strain differences, two additional arrays were used to compare MutaMouse with C57BL/6J DNA. DNA labelled with Cy3 and Cy5 were mixed together for hybridization onto the CGH array.