Arabidopsis seeds translation regulation

This study explores translational regulation during germination by comparing two Arabidopsis thaliana accessions with contrasting dormancy phenotypes: Columbia (Col), which germinates readily, and Cape Verde Islands (Cvi), which exhibits deep dormancy. Using sucrose gradient centrifugation, we isolated monosomal and polysomal fractions from freshly harvested (FH), after-ripened (AR), and imbibed (IM) seeds. In this study, we have compared the mRNAs and associated proteins stored in monosomes or polysomes at the stages of seed maturity, post-ripening and early germination using two Arabidopsis genotypes differing in dormancy level