Mycobacterium tuberculosis Targeted Locus (Loci). Mycobacterium tuberculosis

The study's goal was to evaluate the performance of amplicon sequencing of Mycobacterium tuberculosis (TB) DNA from patient sputum samples using a tabletop next-generation (Next Gen) sequencing technology and automated data analysis for an affordable rapid drug susceptibility testing (RDST) solution (Next Gen-RDST). Our Next Gen-RDST analysis focused on six TB genes associated with drug resistance: eis, rrs, gyrA, rpoB, katG, and inhA. The automated data analysis tool TB Amplicon Sequencing Analysis Pipeline (TB-ASAP) produces a clinically relevant RDST report.