Differential response to Lenalidomide from WT and RUNX1-KO MDS-L del(5q) cells

We used CRISPR/Cas9 system to generate WT Control and RUNX1-KO MDS-L cells isolated as single clones from the parental MDS-L cell line. We have observed that RUNX1-KO MDS-L cells are resistant to Lenalidomide-induced cell death compared to WT Control MDS-L cells We have treated WT and RUNX1-KO MDS-L cells with 1µM Lenalidomide for 24h or 72h and performed expression analysis The experiment consists in eight groups in total. Each group was analyzed with three technical replicates. The groups (per timepoint) are as follows: MDS-L WT DMSO; MDS-L WT LEN; MDS-L KO DMSO; MDS-L KO LEN. We then compared the transcriptional response to LEN within each genotype (MDS-L WT or MDS-L RUNX1-KO).Genes with padj<0.05 we identified as differentially expressed.