ENT1 and cell proliferation in the IVD

Mice lacking equilibrative nucleoside transporter 1 demonstrate progressive calcification of spinal tissues including the annulus fibrosus (AF) of the intervertebral disc (IVD). To identify cellular pathways altered by loss of ENT1, we conducted microarray analysis of AF tissue from wild-type (WT) and ENT1-null mice before calcification (2 months) and associated with calcification (6 months). There are 2 timepoints in this data set, each with a respective WT control. Thus, there are 4 groups total: 2 month WT, 2 month ENT1-null, 6 month WT, and 6 month ENT1-null