MAD2 converted to an inhibitory state via interaction with Mad1

In vitro structural studies have shown that Mad2 undergoes a major conformational change upon binding to Mad1. This conformational change is postulated to activate Mad2 into a high affinity state which can bind and sequester Cdc20 from the APC.

体外结构研究业已表明，Mad2在与Mad1结合后发生显著的构象变化。此构象变化被假定为激活Mad2进入高亲和力状态，进而能够与Cdc20结合并将其隔离于APC中。