Human Prostate Cancer Single-cell RNA Sequencing

6 fresh primary PCa and CRPC/mCRPC specimen included: 3 primary prostate adenocarcinoma (Gleason Score 4+3, both cancer and matched benign prostate tissue) obtained through radical prostatectomy; 2 post-hormonal therapy locally recurrent CRPC with either CRPC-adeno or SCNC histology obtained through transurethral resection of the prostate (TURP); and 1 metastatic CRPC (mCRPC) to pelvic sidewall obtained through surgical resection. Cells were collected from either tumor or benign tissue regions by flow cytometric cell sorting (FACS), and single cell libraries were constructed using 10x GENOMICS platform.