The effect of radiotherapy on the transcriptional profile of proliferating human cells

We have used RNA-sequencing on six different proliferating cell lines consisting of normal human dermal fibroblasts (NHDF), normal human epidermal keratinocytes (NHEK), pericytes (PC), human microvADSCular blood endothelial cells (HMEC), lymphatic endothelial cells (LEC) and adipose derived stem cells (ADSC), subjected to different doses of radiotherapy. Overall design: RNA-seq was performed on proliferating cell lines in duplicate or triplicate under no treatment (0Gy), 5x 2Gy fractions of radiotherapy or 10Gy single dose radiotherapy. Each replicate refers to cells that came from different flasks (i.e. grown separately).