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scNanoSeq-CUT&Tag: a long-read single-cell CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1042935
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We present scNanoSeq-CUT&Tag ,which is based on a long-read sequencing platform. In six human cell lines, we identified regions regulated by H3K4me3,H3K27ac,H3K36me3,H3K27me3,H3K9me3,CTCF and RAD21 modifications. Long fragments (about 4-5Kb) of single-cell ATAC-seq library were enriched and sequenced by Oxford Nanopore Technologies platform. Ends of long CUT&Tag fragments are regarded as chromatin modification signal in downstream analysis.
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2023-11-20
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