Effect of LXR agonist T0901317 on the intestinal transcriptome in mice.. Effect of LXR agonist T0901317 on the intestinal transcriptome in mice.

Mice were treated for three days by oral gavage with the synthetic LXR ligand T0901317 (Cayman chemical, USA) using a dose of 50 mg/kg/day in vehicle (0.5% Carboxy-methylcellulose; 400 cPs (Sigma-Aldrich, USA); 0.2% Tween80 (Sigma-Aldrich, USA)) or solely the vehicle (control group). Mice were anesthetized with isoflurane and euthanized by cardiac puncture followed by cervical dislocation. Intestines were flushed with ice cold PBS and frozen in liquid nitrogen. Overall design: 7 male mice (C57BL/6J): 4 vehicle and 3 T09 treated, RNAseq analysis of the duodenum