Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station

Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes by supplementing GDNF and FGF2 (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and their gene expression profiles were analysed. In this dataset, we include the DNA methylation data obtained from cultured spermatogonia (GS cells) derived from transgenic mouse line C57BL6/Tg14(act-EGFP-OsbY01) that was bred into DBA/2 background. Each group contains 3 biological replicates.