Time series mRNA abundance measurement triggered by ActinomycinD treatment in patient iPSC derived neurons. Time series mRNA abundance measurement triggered by ActinomycinD treatment in patient iPSC derived neurons

We measured bulk mRNA abundance in Ngn2 induced patient derived cortical neurons in synucleinophaty models. ActD shuts off the transcription allowing us to measure mRNA decay. Overall design: Biological samples: SNCA 2 copy neurons and SNCA 4 copy neurons, Treatment: ActD(10 ug/ml), Collection times: 0,1,3,6,12 hour after ActD tretment, Perturbation: SNCA preformed fibril addition(5 ug/ml)