Identification of genes related to male sterile in Prunus sibirica

Stamen development is an important developmental process that directly affects the yield of Prunus sibirica. In this study, the male sterile flower buds and male fertile flower buds of Prunus sibirica were used as materials to performed RNA-Seq analyses to compare transcription differences. The results would provide a theoretical basis for further investigation of the formation mechanism of male sterile flower. Overall design: Male sterile flower buds of clone No. 1 were selected as the experimental group, and male fertile flower buds of clone No. 60 were selected as the control group. Flower bud samples of male sterile clone and male fertile clone were collected from the end of July in 2018 to the beginning of April in 2019. Samples were taken every 10 to 15 days. Every time 10~20 complete flower buds without diseases and insect pests were selected from the upper part of the fruiting branches outside the canopy on the sunny side, which were collected and quickly stored in FAA fixative for making paraffin sections of flower buds. At the same time, three biological replicates of flower buds were collected and rapidly plunged into the liquid nitrogen (-196?). The samples were taken back to the laboratory and stored in an ultra-low temperature freezer(-80°C). Total RNA was extracted from male sterile flower buds and fertile flower buds by RNA extraction kit of Tiangen Biotech (Beijing) Co., Ltd. The RNA librarie sequencing based on Illumina HiSeq platform by paired-end experiments.