Modulation of insulin secretion through RBFOX2-mediated alternative splicing [RNA-seq MIN6]

Recent studies have identified dysregulation of RNA-binding proteins (RBPs) and aberrant mRNA splicing in the onset of diseases including diabetes. Here we investigated the role of RBFOX2 in the pancreatic β cell through the conditional mutation of Rbfox2 in the mouse pancreas (Pdx1:Cre; Rbfox2fl/lf) and RNAi experiments in the mouse insulinoma cell line, MIN6. We then identified the direct targets of RBFOX2 in the mouse β cell transcriptome my eCLIP-Seq in MIN6 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 replicates each for the NT and Rbfox2-KD conditions.