Profiling of 18 - 45 nt small RNAs in the caput epididymal sperm of mice and rats

Total RNAs were extracted from the purified caput epididymal spermatozoa of mouse (C57BL/6, 2 months old) and rats (Sprague Dawley, 450g young adult), and the 18 - 45 nt fraction small RNAs were subjected to library construction and deep sequencing, using Illumina HiSeq 2000. Deep sequencing profiling of 18 - 45 nt small RNAs in the caput epididymal sperm of mouse (C57BL/6, 2 months old) and rats (Sprague Dawley, 450g young adult) by Illumina HiSeq 2000.从纯化后的C57BL/6品系2月龄小鼠以及体重450g的Sprague Dawley品系青年成年大鼠的附睾头精子中提取总RNA，随后对18~45 nt的小分子RNA片段进行文库构建，并利用Illumina HiSeq 2000平台开展深度测序。本数据集通过Illumina HiSeq 2000平台，完成了上述C57BL/6品系小鼠及Sprague Dawley品系大鼠附睾头精子中18~45 nt小分子RNA的深度测序谱分析。