All trans-retinoic acid (ATRA) effect on triple negative (TNBC) and luminal (LumA/LumB) primary breast tumors

We performed whole genome gene expression microarrays on short-term cultures of mammary tumors deriving from diagnostic Tru-cut procedures of 16 patients in order to evaluate transcriptional response to the retinoid. Tissue slices were challenged with vehicle (DMSO) or ATRA 0.1μm for 48 hours. The panel consisted of 11 luminal (7xLumA / 4xLumB) and 5 triple negative (TN) primary tumors. All the LumA and LumB tumors were characterized by >70% ER+ cells.