Overview of EAV nsp1 pseudorevertants described in this study.

aVirus clones were isolated from plaque assays of culture supernatants harvested between 20 h and 24 h post transfection. The number of clones containing each mutation is indicated in brackets.bNumbers indicate the start coordinate of the codon in the EAV genome.cPseudorevertants were reconstructed in the wild-type EAV background, and infectious progeny titers were determined by plaque assays of culture supernatants harvested at 24 h post-transfection. The average titers of three independent experiments are shown.dNA, not applicable.eA silent mutation changing the original alanine codon from GCG to GCC was introduced as a marker mutation upon construction of the ZCH mutant.