Human iPSC-derived renal collecting duct organoids model cystogenesis in ADPKD

Here, establishing expansion cultures of hiPSC-derived ureteric bud tip cells, an embryonic precursor that gives rise to collecting ducts, we succeeded in advancing the developmental stage of collecting duct organoids and showed that all collecting duct organoids derived from PKD1-/- hiPSCs spontaneously develop multiple cysts, clarifying the initiation mechanisms of cystogenesis. Overall design: RNA sequencing analysis for PKD1+/+ and PKD1-/- ureteric bud tip cells cultured for 6 weeks.