Immune repertoire after immunization as seen by next generation sequencing and proteomics

We study the repertoire of immunoglobulin sequences after immunization in a cohort of rats. Animals were immunized with dinitrophenol modified KLH or with recombinant human HuD. In the immune reprtoire, and also in the matching proteomics data, we observe that the animals produce a repertoire that has many shared motifs for those immunized with the same antigen. Cluster analysis allows the samples to be segregated according to the immunogen used.本研究针对一组大鼠队列，分析其经免疫接种后的免疫球蛋白序列组库。实验动物分别以二硝基酚修饰的钥孔血蓝蛋白（KLH）或重组人HuD蛋白进行免疫。在免疫组库及匹配的蛋白质组学数据中，我们观察到：接受同一抗原免疫的动物所产生的序列组库，存在大量共有基序。通过聚类分析，可依据所用免疫原对样本进行区分。