DNA methylation profilings of Blood Cell collected from CELSS 180-day experiment

In this study, we designed a space simulation study, named “4 Subjects 180-Day Controlled Ecological Life Support System (CELSS) Integration Experiment”, which took place in Shenzhen, China, from June to December 2016. In this experiment, four subjects (3 males and 1 female) lived for 180 days in an enclosed simulated cabin, and multiple-sampling-point DNA methylation data was collected to conduct the epigenic analysis. Peripheral whole blood cells were extracted from all 4 subjects on the 12 sampling points (Pre45, Pre15, R2, R30, R60, R75, R90, R105, R120, R150, R175 and Post30 mission day during the experiment). A longitudinal epigenetic profiling of 4 subjects in “4 Subjects 180-Day Controlled Ecological Life Support System (CELSS) Integration Experiment”. 48 samples of blood cell DNA methylation profiling were obtained by Illumina HumanMethylation450 BeadChip, across 12 sampling points during the 180 days mission for all the 4 subjects