Exploring of InDel in bovine <i>PSAP</i> gene and their association with growth traits in different development stages

<i>PSAP</i> (prosaposin) is widely expressed in different organs, and plays an important role in fat deposit. Insertion/Deletion (InDel) is a relatively simple and effective DNA marker. However, the association of molecular marker at different stages of animal development has not received enough attention, especially fat deposition related traits. Therefore, eight cattle breeds were used to explore novel InDels variants within bovine <i>PSAP</i> gene, and to evaluate their effects on growth traits in different development stages. Herein, two novel InDels (P5:NC037355.1g.27974439-27974440 ins AGTGTGGTTAATGTCAAC and P8:NC037355.1g.27980734-27980752 del GTCAAAAAATCAGGGGAAAC) within the bovine <i>PSAP</i> gene were found, and their association with growth traits in different development stages were analyzed. Interestingly, the dominant genotype was different in different development stages both in NY cattle and JX cattle for daily gain and body weight. <i>PSAP</i> Gene expression patterns were analyzed in this study, high expression in the middle stage of adipocytes differentiation suggests that it plays a certain role in fat development. It reveals that InDels could affect phenotype in different development stages, which depend on the expression pattern of the host gene and their function in different tissues. These findings could provide a new way for molecular marker studies in bovine breeding and genetics.鞘脂激活蛋白原（prosaposin，PSAP）在多种器官中广泛表达，且在脂肪沉积过程中发挥重要作用。插入/缺失（Insertion/Deletion，InDel）是一类较为简单高效的DNA分子标记。然而，针对动物发育不同阶段的分子标记关联研究尚未受到足够关注，尤其是脂肪沉积相关性状。因此，本研究选取8个牛品种，对牛PSAP基因内的新型InDel变异进行探究，并评估其在不同发育阶段对生长性状的影响。本研究共发现牛PSAP基因内的2个新型InDel变异（P5:NC037355.1g.27974439-27974440 插入AGTGTGGTTAATGTCAAC，以及P8:NC037355.1g.27980734-27980752 缺失GTCAAAAAATCAGGGGAAAC），并分析了这两个变异与不同发育阶段生长性状的关联。有趣的是，在NY牛和JX牛群体中，针对日增重和体重性状，不同发育阶段的优势基因型存在差异。本研究还对PSAP基因的表达模式进行了分析：其在脂肪细胞分化中期呈现高表达，提示该基因在脂肪发育过程中发挥一定作用。该研究结果表明，InDel变异可通过宿主基因的表达模式及其在不同组织中的功能，影响动物不同发育阶段的表型性状。上述研究结果可为牛育种与遗传学领域的分子标记研究提供新的思路。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于牛PSAP基因中的插入/缺失（InDel）变异研究，旨在探索这些变异与不同发育阶段生长性状的关联。研究发现两个新的InDel变异，并分析表明它们在不同发育阶段对日增重和体重等性状有影响，且PSAP基因在脂肪细胞分化中期高表达，提示其在脂肪发育中起作用。这些结果为牛育种和遗传学中的分子标记应用提供了新的视角。