Replication Data for: The magic of mushrooms: Psilocybin influences behaviour in the mangrove rivulus, Kryptolebias marmoratus

The purpose of this study was to determine if the psychoactive compound, psilocybin could induce calming effects and reduce social aggression and activity levels in a mangrove fish. We socially stimulated fish using pairs of size-matched fish from different isogenic lineages and compared baseline social behaviour following a waterborne dose of psilocybin. We measured a variety of behaviours such as i) time spent moving (activity), ii) frequency of head-on and iii) lateral displays, iv) time spent in head-on and v) lateral displays, vi) frequency of mesh barrier zone entries, vii) time spent in mesh barrier zone, viii) frequency of swimming bursts and ix) time spent interacting. We also performed analytical measurements using LC-MS to determine the concentration of psilocybin and psilocin within the fish.