The original sequencing data used in this study are provided.. RNA-Sequencing data
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB65311
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The obtained cardiac tissue RNA was extracted to construct a gene library and then subjected to Illumina high-throughput sequencing. The poly-N, adapter and low-quality reads contained in the original data were deleted to obtain clean reads to ensure the quality and reliability of downstream data analysis; then the comparison software HISAT2 (The Johns Hopkins University, Baltimore, Maryland, United States) was used to compare The obtained Clean Reads were compared with mouse genes, and the number of reads of each gene was calculated according to the location of the reference genome; the Feature Counts tool of subread software was used to quantify the gene expression level; DESeq2 software was used to analyze the differential expression between groups, a corrected p-value of 0.05 and an absolute fold change of two were set as the threshold for significantly differential expression.
创建时间:
2023-10-22



