Primers used for constructions of His6-tagged PGK and PGK truncationsa.
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aAll regions were amplified using the respective mutagenic forward and reverse primers, as required.bIntroduced EcoRI, HindIII and NdeI sites underlined.cupstream position of nucleotide from the adenine nucleotide of the starting ATG codon.*11 nucleotides are downstream the stop codon localized at position 1239.
创建时间：
2015-12-02
