Characterizing the relation of functional and Early Folding Residues in protein structures using the example of aminoacyl-tRNA synthetases

Proteins are chains of amino acids which adopt a three-dimensional structure and are then able to catalyze chemical reactions or propagate signals in organisms. Without external influence, many proteins fold into their native structure, and a small number of Early Folding Residues (EFR) have previously been shown to initiate the formation of secondary structure elements and guide their respective assembly. Using the two diverse superfamilies of aminoacyl-tRNA synthetases (aaRS), it is shown that the position of EFR is preserved over the course of evolution even when the corresponding sequence conservation is small. Folding initiation sites are positioned in the center of secondary structure elements, independent of aaRS class. In class I, the predicted position of EFR resembles an ancient structural packing motif present in many seemingly unrelated proteins. Furthermore, it is shown that EFR and functionally relevant residues in aaRS are almost entirely disjoint sets of residues. The Start2Fold database is used to investigate whether this separation of EFR and functional residues can be observed for other proteins. EFR are found to constitute crucial connectors of protein regions which are distant at sequence level. Especially, these residues exhibit a high number of non-covalent residue-residue contacts such as hydrogen bonds and hydrophobic interactions. This tendency also manifests as energetically stable local regions, as substantiated by a knowledge-based potential. Despite profound differences regarding how EFR and functional residues are embedded in protein structures, a strict separation of structurally and functionally relevant residues cannot be observed for a more general collection of proteins.