Flux balance analysis of GSMN-ML on glucose and glycerol as carbon sources and gene essentiality predictions.

File shows flux variability analysis and gene essentiality analysis for the model. Two media p-files was set for this test. One is to use glycerol as only carbon source and the other is to use glycerol as only carbon source and the other necessary molecules. Table 'FVA' shows results for all reactions with maximized biomass. Table 'media' shows media settings, those substrates whose lower-bound < 0 are allowed to be uptaken. Table 'metabolite_name' shows metabolite IDs and their names. Table 'KO_gene' shows gene essentiality analysis results on the media set in 'Media'. GRateKO: growth rate when the gene knocked out. Ratio: calculated as GRateKO/GRateWT(wildType). Genes are considered as essential If Ratio < 0.01 (marked red).
(XLSX)