NcdY485 mutant motility.

Ensemble microtubule gliding assays were performed in HEM (10 mM HEPES pH 7.2, 1 mM EGTA, 1 mM MgCl2) using the same volume and concentration of anti-GST antibodies to attach the motors to the coverslip. NaCl was added to the assays to the indicated concentrations to optimize motor-microtubule binding and gliding velocity; higher NaCl concentrations than those shown resulted in partially bound or no bound microtubules. Microtubules were not tracked if they were curved or had ends that were unfocused or out of the field, or were recorded *Mean±SEM.ƒUnless otherwise indicated, coverslips were precoated with motor before adding a further aliquot of motor for the assay.