Mouse feeder-free alveolar organoids treated by bleomycin. Mouse feeder-free alveolar organoids treated by bleomycin

To investigate the direct effect of bleomycin on alveolar epithelium, feeder-free mouse alveolar organoids were treated by bleomycin (100μM) for 48 hours in vitro and then analyzed. Overall design: Alveolar organoids were made from primary alveolar epithelial cell type 2 (GFP+ in FACS) isolated from tamoxifen-treated Sftpc-creERT2; Rosa26-mTmG mice (male, 9wks). Comparative gene expression profiling analysis of RNA-seq data between bleomycin-treated and untreated mouse alveolar organoids.