Identification of bivalent genes in CD14+ Monocytes

Identification of bivalent genes, marked with both H3K4me3 and H3K27me3, in CD14+ Monocytes. Used previously published data sets for histone marks GSM1003536 and GSM1003564. We used previously published ChIP-Seq data from ENCODE in CD14+ cells for histone markd H3K4me3 (GSM1003536) and H3K27me3 (GSM1003564) from the data series GSE29611 and called peaks with MACS2 with default parameters with the exception of H3K27me3 with the additional --broad parameter. Intersecting peaks were identified with the bedtools intersect tool. Processed data file generated for GSM1003536 and GSM1003564: CD14+_H3K27me3_H4k4me3_Intersection.bed.gz.