Gene signature from lung tissue of e-cigarette users

Background: E-cigarette popularity is on the rise in youth and young adults, with mounting concerns regarding the long-term safety of these devices. Cell culture and animal models have highlighted the damaging potential of e-cigarettes, but to date there is a lack of data from human lung tissue to corroborate these findings. Methods: Using human lung tissue obtained during a bullectomy in young adults, we performed RNA-sequencing to uncover e-cigarette related changes to the human lung transcriptome. Information on e-cigarette use habits was collected via questionnaire. Samples included in study: S1, S2, S3, S4, S5, S7, S9, S10, S12, S13, S14, S16, S17, S18, S19, S21 Lung tissue collected from people undergoing surgery to repair a spontaneous pneumothorax. February 2025: Submitter indicated there was an error in the labelling of some of the samples. The error has been amended.