File S1 - Genetic and Metabolite Diversity of Sardinian Populations of Helichrysum italicum

Supporting figures and tables. Figure S1, Admixture proportions at K = 2 of 294 H. italicum individuals sampled from 50 populations. A Bayesian clustering analysis was performed using STRUCTURE and TESS software. The highest likelihood run was identified based on minimum values for ΔK [26] and DIC [27], respectively. Figure S2, Pairwise Fst between the AFLP-based clades identified at A) K = 4 and B) K = 10. At K = 4, clades A and B were the most divergent, but at K = 10, they were replaced by clades G and I. Figure S3, The identification of AFLP loci putatively under selection loci using Bayescan 1.0 software [29]. Each point corresponds to a single AFLP fragment. Fst is plotted against the log10 of posterior odds. The vertical dashed line indicates the chosen threshold value of 2.0. Figure S4, Representative chromatogram of the Essential oil of H. italicum. The main peak in the chromatogram represents neryl acetate. Table S1, Location of the meteorological stations, in terms of their latitude, longitude, elevation and distance from the sea. Table S2, Soil and habitat description. For each population the collection site was described for habitat, CORINE Biotope code (CB) and soil. Habitats and soils description is shown the corresponding columns. The habitats as well as the soils were divided in groups with similar characteristics. Fourteen CB groups (I to XIV) and ten soil types (1–10) were identified; missing data are indicated as n.d. Table S3, The first five PCs explaining >90% of the variation in climate, reduced from a set of 48 climate parameters. Significant correlations between each PC and individual parameters are indicated in bold. Air temperature and rainfall amounts are annual averages. Table S4, Coefficient of membership (Q) with the major clades of individual accessions of H. italicum. A) K = 2, B) K = 4, C) K = 10. Table S5, Contingency analysis for habitat and soil type with respect to AFLP-based clades at K = 2 and K = 4. The analysis was repeated with a restricted set of habitat and soil types. Table S6, The monoterpene fraction of H. italicum essential oil. Table S7, The oxygenated monoterpene fraction of H. italicum essential oil. Table S8, The sesquiterpene fraction of H. italicum essential oil. Table S9, The oxygenated sesquiterpene fraction of H. italicum essential oil. (RAR)